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VOLUME 6 , ISSUE 1 ( January-April, 2012 ) > List of Articles

RESEARCH ARTICLE

An In Vitro Microbiological Study Evaluating the Efficacy of Soluneem (A Water Soluble Neem Formulation from Azadirachta Indica) Against Periodontopathic Microorganisms

A Suchetha, R Vijayendra, G Bharwani Ashit

Citation Information : Suchetha A, Vijayendra R, Bharwani Ashit G. An In Vitro Microbiological Study Evaluating the Efficacy of Soluneem (A Water Soluble Neem Formulation from Azadirachta Indica) Against Periodontopathic Microorganisms. J Oral Health Comm Dent 2012; 6 (1):4-9.

DOI: 10.5005/johcd-6-1-4

License: NA

Published Online: 01-05-2015

Copyright Statement:  NA


Abstract

Objectives

To evaluate the efficacy of Soluneem (a water soluble formulation from the neem seed kernel from Azadirachta Indica containing Azadirachtin) as an antimicrobial agent and the effective concentration of Soluneem required to inhibit periodontopathic bacteria and to compare it with a known antiplaque agent chlorhexidine (0.2%) in vitro.

Material and Methods

The subgingival plaque samples from a total of 20 sites from 5 patients (4 sites per patient) was taken with a probing pocket depth of >”5mm. Subgingival plaque samples were collected, cultivated, and incubated anaerobically as per the standard procedure. Further subculturing was done to obtain pure growth. Various biochemical tests were used for identifying each organism as per the standard protocol. The sensitivity to the varying concentrations of neem like 100%, 50%, 25%, 12.5%, 6.25%, 3.125% and 1.5625% and 0.2% Chlorhexidine solution was tested using the Modified Disc diffusion method. The sensitivity was tested by measuring the zones of inhibition comparing with the known standard diameter.

Results

The organisms isolated and percentage of identification rate from total of five patients was 60% for Bacteroids fragilis, 40% for Bacteroids distasonis, 20% for Prevotella corporis, 20% for Prevotella melaninogenica, and 20% for Peptostreptococus species. Chlorhexidine 0.2 % was effective against micro organisms cultivated and isolated in this study. However Soluneem was not effective against the organisms cultivated in this study.

Conclusion

Soluneem had no effect on the growth of any of the isolated organisms. Further studies testing soluneem against other putative periodontal pathogens with Azadirachtin are warranted.


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