Aim: This study aimed to assess the knowledge and anxiety levels of dentists and pregraduate dental students about COVID-19 in Turkey. Materials and Methods: A three-part online questionnaire was used to collect data. Questionnaire 1 consisted of 10 demographic questions. In questionnaire 2, there were 10 multiple-choice questions, including various COVID-19 knowledge. In questionnaire 3, participants were tested by a self-evaluation test as Worry and Anxiety Questionnaire (WAQ). Results: A total of 1002 respondents had completed the questionnaires. The majority of the participants exhibited very good knowledge. Worry and anxiety scores differ by gender, institution, and specialty (p < 0,001). There was no significant relationship between knowledge level and stress level (p > 0,050). Conclusion: Female dentists, pediatric dentists, and dentists working in public hospitals in Turkey were found to be significantly more stressed. The level of knowledge was generally high and no correlation was established by the level of anxiety. Clinical Significance: In this global pandemic process, it is known that all dentists’ problems and needs are common, and the effects of the disease on the dental profession can be globalized. It is crucial in the face of the anxiety and stress demonstrated by dentists during COVID-19 that mental coping mechanisms and strategies generated to remain calm and function efficiently.